moligoTECHNOLOGIESPartner with us

Every strand perfect.
Any strand modified.

mRNA moment for single stranded DNA.

Explore modified DNADiscuss your project
Conceptual molecular illustration

01 / Co-development

Modified ssDNA.A partnership programme.

Co-develop long modified ssDNA with our scientists. Together, we define the application, assess chemistry feasibility and agree the analytical and biological work. Many of the modifications which enabled new short DNA therapeutics and RNA molecules can be applied to long single-stranded DNA. Modification technology is available to selected partners. Participation and scope are agreed for each partnership.

Explore the partnership programme
Close view of automated dispensing needles and sample vials

Why our technology

Build chemistry into the strand.

Compare the production approaches

End adaptors protect the ends. A modified oligonucleotide adds a local patch. Moligo incorporates modified nucleotides during synthesis, distributing chemistry throughout long ssDNA.

This expands what can be investigated at kilobase length: composition across the molecule, alongside linear or circular format. The programme brings synthesis, characterisation and application testing into one collaboration.

Incorporation is global but tunable, with the degree of modification controlled by the ratio of modified to natural nucleotides. The precedent is modified mRNA, where uridine is replaced throughout the transcript rather than at selected positions.

The strand can also carry built-in clickable sites for ligand conjugation, so chemistry is not limited to the nucleotides themselves.

Karikó et al., Immunity 2005. Nobel Prize in Physiology or Medicine 2023.

Published research

Published short-donor study: internal modification added 26 HDR points.

Read the evidence and its limits

In a published short-donor study, internal chemical modification gave 41 ± 15% HDR, versus 24 ± 6% for end-modified and 15 ± 5% for unmodified donors.

The experiment used airway cells, the CFTR locus and the DNA-PK inhibitor AZD-7648.

Kanke et al., Nucleic Acids Research, 2024. Kanke et al. tested short donors. Moligo carries the principle of internal modification to kilobase-length DNA through its partnership programme. Separately, Moligo’s work with Cellectis evaluated circular ssDNA as a non-viral donor in blood stem and progenitor cells.

Discuss your DNA.

Tell us about your sequence, application and the chemistry you want to investigate.

Talk to our scientists